Useful Links & Tools
Last 5 Products Viewed
FG0100
Sigma
Free Glycerol Determination Kit
1 kit sufficient for 1000 reactions
| Synonym: | Triglyceride and Free Glycerol Kits and Reagents |
Description
| Application | The triglyceride and free glycerol kits are for the quantitative determination of glycerol, total triglycerides or free triglycerides. |
| Linkage | In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed. |
| The Free Glycerol Determination Kit measures free, endogenous glycerol using coupled enzyme reactions and does not include initial lipase hydrolysis. Glycerol is phosphorylated by adenosine-5'-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5'-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to the free glycerol concentration of the sample. |
Properties
| usage | kit sufficient for 1000 reactions |
| storage temp. | 2-8°C |
Safety
| Hazard Codes | Xn |
| Risk Statements | 21/22-32-36 |
| Safety Statements | 7-26-36/37 |
